一、基础信息
货号：RCP-30-5A
英文名称：SPHERO™ Rainbow Calibration Particles, 8 Peaks, 3.0-3.4 μm
中文名称：SPHERO™彩虹校准微球（8峰，3.0-3.4微米）
品牌：Spherotech
规格：10?个/mL，5mL/瓶
中国代理商：上海起发实验试剂有限公司
Spherotech成立于1992年，总部位于美国伊利诺伊州，是微球产品制造商，专注于为生物医学、诊断和药物开发领域提供均一化微球解决方案。其产品以高精度、高稳定性和广泛适用性著称，被全球科研机构、生物技术公司及医疗机构广泛采用。
二、产品原理
SPHERO™彩虹校准微球通过多色荧光标记技术实现对仪器的全面校准。每个微球内部均匀嵌入多种荧光染料（如FITC、PE、PE-Cy5、APC等），形成8个荧光强度梯度的峰。这些荧光染料的激发波长覆盖365-650nm，可适配主流流式细胞仪的激光系统（如488nm、561nm、633nm等）。
其核心原理包括：
1. 多通道同步校准：
单一微球可同时校准流式细胞仪的前向散射（FSC）、侧向散射（SSC）及多个荧光通道（如FL1-FL5），通过检测8个荧光峰的位置和强度，验证仪器的灵敏度、线性度和分辨率。
2. 荧光稳定性设计：
染料通过物理包埋而非表面偶联的方式固定在微球内部，避免了外界环境（如pH、温度、酶解）对荧光信号的干扰，确保长期储存和重复使用时的信号一致性。
3. 对数放大器线性验证：
微球的荧光强度呈对数分布，可通过配套的Excel模板（如SPHERO™ PMT Quality Control Template）生成校准曲线，定量评估仪器对数放大器的线性误差，确保检测结果的准确性。
三、解决实验中的关键问题
1. 仪器标准化与质量控制：
- 流式细胞仪的光路校准：通过FSC和SSC信号调整液流稳定性和激光对齐，确保细胞大小和颗粒度检测的准确性。
- 荧光通道灵敏度验证：检测微球的荧光强度阈值，确认仪器对弱信号的检测能力，避免因PMT电压设置不当导致的假阴性结果。
- 线性度评估：验证对数放大器在宽动态范围内的线性响应，确保高表达和低表达样本的信号均能准确区分。
2. 实验数据的可比性与重复性：
- 多实验室间数据标准化：通过统一使用SPHERO™彩虹校准微球，可消除不同仪器和实验条件对结果的影响，实现跨平台数据的可比性。
- 长期实验质量跟踪：定期校准可监测仪器性能变化，及时发现光路偏移、激光衰减等问题，避免实验结果的系统性偏差。
3. 复杂实验设计的优化：
- 荧光补偿设置：通过微球的多色荧光特性，快速建立补偿矩阵，减少因荧光光谱重叠导致的信号干扰。
- 多色实验通道匹配：根据微球的荧光发射谱，合理选择荧光染料组合，提高实验检测维度。
四、核心优势
1. 多用途兼容性：
- 适用于主流流式细胞仪品牌（如BD、Beckman Coulter、Cytek等），支持从科研级到临床级仪器的校准需求。
- 除流式细胞仪外，还可用于荧光显微镜、共聚焦显微镜等设备的光路校准和荧光强度标准化。
2. 高稳定性与可靠性：
- 荧光信号在2-8℃储存条件下可稳定保存12个月以上，且耐受反复冻融（至少10次），降低实验成本。
- 微球粒径均一性高（CV<2%），确保检测结果的重复性和统计学意义。
3. 操作简便与经济性：
- 即用型设计，无需复杂预处理，直接取1-2滴微球悬液稀释后即可上机检测。
- 每瓶可进行约50-100次校准（根据仪器进样量调整），单次实验成本显著低于同类产品。
4. 技术支持与资源丰富：
- 提供详细的技术文档（如STN-8、STN-14）和操作视频，指导用户完成校准流程及数据分析。
- 配套Excel模板可自动生成校准报告，简化数据处理步骤。
五、客户群体与典型应用
1. 科研领域：
- 免疫分析：T细胞亚群分型、细胞因子检测、抗体效价评估等实验中，确保流式细胞仪的灵敏度和线性度。
- 细胞生物学：干细胞分化研究、细胞凋亡检测、细胞周期分析等，保证实验数据的准确性和可重复性。
- 癌症研究：循环肿瘤细胞（CTC）计数、肿瘤微环境分析等，对稀有细胞的检测精度要求高。
2. 临床诊断与IVD：
- 血液学检测：白血病免疫分型、血小板功能分析等，需严格遵循临床实验室标准化流程。
- 感染性疾病诊断：HIV病毒载量监测、结核杆菌抗原检测等，确保检测结果的一致性和合规性。
3. 工业质检与生物技术：
- 生物制药：重组蛋白表达分析、病毒载体滴度测定等，对仪器的稳定性和准确性要求很高。
- 纳米材料表征：纳米颗粒粒径分布检测、荧光标记效率评估等，利用微球的荧光特性进行定量分析。
六、订货信息
1. 产品规格：
- 货号RCP-30-5A：8峰，3.0-3.4μm，10?个/mL，5mL/瓶
- 其他可选规格：
- RCP-30-5：6峰，3.0-3.4μm，5mL
- RCP-20-5：4峰，1.8-2.2μm，5mL
- 超彩虹系列（Ultra Rainbow）：适用于紫外和远红外通道校准
2. 储存条件：
- 2-8℃避光保存，避免冷冻。未开封状态下有效期为12个月，开封后建议6个月内使用完毕。
3. 订货渠道：
- 中国代理：上海起发实验试剂有限公司
4. 注意事项：
- 使用前需充分混匀微球悬液，避免因沉淀导致的浓度不均。
- 校准过程中需使用过滤后的去离子水或鞘液，防止杂质干扰检测结果。
- 如发现微球悬液出现浑浊或沉淀，应停止使用并联系供应商更换。
关键词：Spherotech流式细胞仪校准微球,RCP-30-5A,8峰校准微球,3.0-3.4μm微球,荧光校准微球,流式质控微球,多峰校准微球,Ultra Rainbow微球,流式细胞术校准,荧光补偿微球,生物医学微球,诊断用微球,聚苯乙烯微球,流式细胞仪比对微球,尺寸标准微球,荧光强度校准,线性校准微球,灵敏度校准微球,流式细胞仪阈值设定,细胞分选校准,抗体包被微球,磁性微球,乳胶微球,流式细胞术耗材,科研用微球,临床诊断微球,流式细胞仪维护,微球定制,Spherotech品牌微球
更多产品信息，请联系中国区域代理商：上海起发实验试剂有限公司
如果您对任意产品或其他品牌感兴趣，可通过“在线留言"功能留下您的需求
（注：本文内容基于品牌公开资料及行业常规信息整理，具体以品牌信息文档为准。）
??Spherotech热卖产品
货号 | 品名 | 规格 | 品牌 |
RCP-30-5A | Rainbow Calibration Particles, 8 peaks, 1E7/mL, 3.0-3.4µm, 5mL | 5mL | Spherotech |
URFP-30-2 | UltraRainbow Fluorescent Particles 1E7/mL 3.0-3.4 um | 2ml | Spherotech |
NVP-60-5 | Neutravidin Polystyrene Particles 0.5％ w/v 6.0-8.0 um 5 mL | 5mL | Spherotech |
MPFc-60-5 | Gt anti-Ms IgG (Fc) Polystyrene Particles, 0.5％w/v, 6.0-8.0µm, 5mL | 5ml | Spherotech |
URCP-38-2K | UltraRainbow Calibration Particle Kit, 3.8 +/- 0.2µm,2E6/mL, 1x3mL (blank) & 1E7/mL, 1x2mL (fluorescent) | 1x3mL | Spherotech |
SVP5-60-5 | Streptavidin Polystyrene Particles, 5%w/v, 6.0-8.0µm, 5mL | 5mL | Spherotech |
SVP-60-5 | Streptavidin Polystyrene Particles, 0.5％w/v, 6.0-8.0μm | 5mL | Spherotech |
C62930 | Calibration Beads for MoFlo Astrios, 1E7/mL, 3.0-3.4µm, 2 mL | 2mL | Spherotech |
HUP-60-5 | Gt anti-Hu IgG(H&L) Polystyrene Particles, 0.5％w/v, 6.0-7.9μm, 5mL | 5mL | Spherotech |
RCP-30-5 | Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-30-5) | 5mL | Spherotech |
RPFc-60-5 | Goat anti-Rabbit IgG (Fc) Coated Polystyrene Particles 0.5% w，6.0-8.0µm | 5 mL | Spherotech |
DIGP-20-2 | Anti-Digoxigenin Coated Polystyrene Particles, 2.0- 2.4µm, 0.1％w/v, 2mL | 2mL | Spherotech |
ACBP-70-10 | AccuCount Blank, 10^6/mL, 7.0-7.9μm, 10mL | 10mL | Spherotech |
NVM-20-5 | NeutrAvidin Coated Magnetic Particles | 5mL | Spherotech |
NFPPS-52-4K | NANO FLUORESCENT SIZE STANDARD, FLOW CYTOMETRY GRADE, YELLOW, NFPPS系列 | 4x5mL | Spherotech |
URQP-38-6K | Ultra Rainbow Quantitative Particle Kit | 6x2mL | Spherotech |
urfp-100-2 | ULTRARAINBOW FLUORESCENT PARTICLES, URFP系列超级彩虹荧光微球 | 2ml | Spherotech |
SVP-50-5 | Streptavidin Polystyrene Particles, 0.5%w/v, 5.0-5.9µm, 5mL | 5mL | Spherotech |
SVP-40-5 | Streptavidin Coated Polystyrene Particles 0.5％ w/v 4.0-4.9 μm | 5mL | Spherotech |
SVP-10-5 | Streptavidin Polystyrene Particles, 1%w/v, 1.0-1.4um, 5mL | 5mL | Spherotech |
SVP-08-10 | Streptavidin Polystyrene Particles 1％ w/v 0.7-0.9um | 10mL | Spherotech |
SVM-025-5H | Streptavidin Magnetic Particles, High Iron, 0.5％ w/v, 0.2-0.39 um, 5 mL | 5mL | Spherotech |
ACFP-50-5 | AccuCount Fluorescent Particles, 10^6/mL, 5.0-5.9µm, 5mL | 5mL | Spherotech |
MPFc-30-5 | Gt anti-Ms IgG (Fc) Polystyrene Particles, 0.5％w/v, 3.0-3.9µm, 5mL | 5mL | Spherotech |
PPS-6K | Polystyrene Particle Size Standard Kit, Flow Cytometry Grade, 2.5E6/mL, 6x5mL, 2.0-2.4µm, 3.0-3.4µm, 5.0-5.9µm, 7.0-7.9µm, 8.0-12.9µm, & 13.0- 17.9µm | 6x5mL | Spherotech |
CMIGP-70-3K | COMPtrol Gt anti-Ms Ig(H&L) Particles Kit, 7.0-7.9µm., 3X5mL | 3X5mL | Spherotech |
CMX-200-10 | Carboxyl Magnetic Particles, Crosslinkled, 1%w/v, 18.0-22.9µm, 10mL | 10mL | Spherotech |
FP-00556-2 | Fluorescent Particles, Nile Red, 1%w/v, 0.04-0.06µm, 2mL | 2mL | Spherotech |
CMX-300-1000 | Carboxyl Magnetic Particles, Crosslinked,1％ w/v, 25-37 um, 1000 mL | 1000mL | Spherotech |
SVP-15-5 | Streptavidin Polystyrene Particles 1％ w/v 1.5-1.9um 5 mL | 5mL | Spherotech |
CP-10-10 | Carboxyl Polystyrene Particles, 5％w/v, 1.0-1.4µm, 10mL | 10mL | Spherotech |
DDCP-70-2 | Drop Delay Calibration Particles | 2mL | Spherotech |
DIGP-40-2 | Anti-Digoxigenin Coated Polystyrene Particles, 4.0- 4.9um, 0.1%w/v, 2mL | 2mL | Spherotech |
PGP-08-5 | Protein G Polystyrene Particles, 1%w/v, 0.7-0.9µm, 5mL | 5mL | Spherotech |
PAP-60-5 | Protein A Polystyrene Particles, 0.5%w/v, 6.0-8.0um, 5mL | 5mL | Spherotech |
PAP-40-5 | Protein A Polystyrene Particles, 0.5%w/v, 4.0-4.9um, 5mL | 5mL | Spherotech |
PAP-08-5 | Protein A Polystyrene Particles, 1%w/v, 0.7-0.9µm, 5mL | 5mL | Spherotech |
PGP-30-5 | Protein G Polystyrene Particles, 0.5％w/v, 3.0-3.4µm, 5mL | 5mL | Spherotech |
PM-40-10 | Polystyrene Magnetic Particles, 2.5%w/v, 4.0-4.5µm, 10mL | 10mL | Spherotech |
PP-08-10 | polystyrene particles，5％w/v，0.7-0.9um | 10ml | Spherotech |
PP-100-10 | Polystyrene Particles, 2.5%w/v, 8.0-12.9um, 10mL | 10mL | Spherotech |
PP-20-10 | Polystyrene Particles, 5%w/v, 2.0-2.4µm, 10mL | 10mL | Spherotech |
PP-30-10 | Polystyrene Particles, 5%w/v, 3.0-3.4µm, 10mL | 10mL | Spherotech |
PP10-10-10 | Polystyrene Particles, 10％w/v, 1.0-1.4µm, 10mL | 10mL | Spherotech |
MP-60-5 | Gt anti-Ms IgG (H&L) Polystyrene Particles, 0.5％w/v, 6.0-8.0µm, 5mL | 5mL | Spherotech |
ACBP-20-10 | AccuCount Blank Particles | 10mL | Spherotech |
MMSFc-40-10 | Gt anti-Ms IgG (Fc) Magnetic Particles, Smooth, 1％w/v, 4.0-5.0um, 10mL | 10mL | Spherotech |
PPX-600-10 | Polystyrene Particles, Crosslinked, Research Grade, 5%w/v, 53.0-69.0µm, 10mL | 10mL | Spherotech |
MM-40-10 | Gt anti-Ms IgG (H&L) Magnetic Particles, 1％w/v, 4.0-4.9μm, 10mL | 10mL | Spherotech |
HUP-100-5 | Gt anti-Hu IgG(H&L) Polystyrene Particles, 0.5%w/v, 10.0-14.0µm, 5mL | 5mL | Spherotech |
HMX-10-10 | Gt anti-Hu IgG (H&L) Magnetic Particles, Crosslinked, 0.5%w/v, 1.0-2.0um, 10mL | 10mL | Spherotech |
FSVM-8058-2 | Streptavidin Fluorescent Magnetic Particles, Pink, 0.1％w/v, 8.0-9.9μm, 2mL | 2mL | Spherotech |
FSVM-2058-2 | Streptavidin Fluorescent Magnetic Particles, Pink, 0.1％w/v, 2.0-2.9μm, 2mL | 2mL | Spherotech |
FSVM-02556-2 | Streptavidin Fluorescent Magnetic Particles, Nile Red, 0.1％w/v, 0.2-0.39μm, 2mL | 2mL | Spherotech |
FPS-15057 | Fluorescent Particle Slide, Rainbow, 13.0-17.9µm | 13.0-17.9um | Spherotech |
FPS-10057 | Fluorescent Particle Slide, Rainbow, 10.0-14.0µm | 10.0-14.0um | Spherotech |
RCP-30-5A-8 | Rainbow Calibration Particles, 8 peaks, 1E7/mL, 3.0-3.4µm, 5mL | 5mL | Spherotech |
RFP-30-5A | Rainbow Fluorescent Particles, Mid-Range, 1E7/mL, 3.0-3.4µm, 5mL | 5mL | Spherotech |
FP-0256-2 | Fluorescent Particles, Nile Red, 1%w/v, 0.1-0.3µm, 2mL | 2mL | Spherotech |
RPFc-08-20 | Gt anti-Rb IgG (Fc) Polystyrene Particles, 0.25%w/v, 0.7-0.9µm, 20mL | 20mL | Spherotech |
RTPFC-60-5 | Goat anti-Rat IgG (Fc) Coated Polystyrene Particles, Cross-adsorbed, 0.5%w/v, 6.0-8.0um, 5mL | 5mL | Spherotech |
FH-3056-2 | Fluorescent Particles, Nile red, High Intensity, 1%w/v, 2.5-4.5µm, 2mL | 2mL | Spherotech |
SVFM-20-5 | Streptavidin Ferromagnetic Particles, 1%w/v, 2.0-2.9um, 5mL | 5mL | Spherotech |
SVFP-0556-5 | Streptavidin Fluorescent Particles, Nile Red, 0.1％w/v, 0.4-0.6μm 5mL | 5mL | Spherotech |
SVFP-6056-5 | Streptavidin Fluorescent Particles, Nile Red, 0.1%w/v, 5.0-7.9µm, 5mL | 5mL | Spherotech |
SVFP-6062-5 | Streptavidin Fluorescent Particles, Purple, 0.1%w/v, 5.0-7.9µm, 5mL | 5mL | Spherotech |
DIGP-08-2 | Anti-Digoxigenin Coated Polystyrene Particles, 0.7- 0.9um, 0.1%w/v, 2mL | 2mL | Spherotech |
CMX-300-1 | Carboxyl Magnetic Particles, Crosslinked, 1％ w/v，25-37um | 2x 1mL Samples | Spherotech |
URCP-100-2 | UltraRainbow Calibration Particles, 6 peaks, 8.0-12.9um, 1E7/mL, 2mL | 2mL | Spherotech |
TM-40-10 | Biotin Magnetic Particles, 1％w/v, 4.0-4.5µm, 10mL | 10mL | Spherotech |
TFP-0556-5 | Biotin Fluorescent Particles, Nile Red, 0.1％ w/v, 0.4-0.6 um, 5 mL | 5mL | Spherotech |
URCP-100-2-1 | Ultra Rainbow Calibration Particle, Peak 1, 8.0 - 12.9 um, 1E7/mL | 2mL | Spherotech |
CM-025-10H | Carboxyl Magnetic Particles, High Iron, 1.0％ w/v, 0.1-0.39 um, 10 mL | 10mL | Spherotech |
CFM-80-5 | Carboxyl Ferromagnetic Particles, 1％w/v, 8.0-8.9µm, 5mL | 5mL | Spherotech |
CM-05-10H | Carboxyl Magnetic Particles, High Iron, 1.0％w/v, 0.4-0.69µm, 10mL | 10mL | Spherotech |
URCP-100-2-6 | SPHERO Ultra Rainbow Calibratior Particles,Peak 6,10.4 um, 2 mL | 2mL | Spherotech |
URCP-100-2-8 | SPHEROm Ultra Rainbow CalibrationParticles，Peak 8,10.6 um, | 2mL | Spherotech |
CM-200-10 | Carboxyl Magnetic Particles, 1％w/v, 18.0-22.9µm, 10mL | 10mL | Spherotech |
CFM-20-10 | Carboxyl Ferromagnetic Particles, 1％w/v, 2.0-2.9µm, 10mL | 10mL | Spherotech |
CM-60-10 | Carboxyl Magnetic Particles, 2.5％w/v, 6.0-8.0µm, 10mL | 10mL | Spherotech |
URFP-02-2 | Ultra Rainbow Fluorescent Particles, ~1E10/mL, 0.1-0.3µm, 2mL | 2mL | Spherotech |
AUP5-002-20 | Citrate Gold Particles, 5.0nM, 15-20nm, 20mL | 20mL | Spherotech |
CMPAK-4068-6K | Carboxyl Magnetic Blue Particle Array Kit, 6 Peaks, 1E7/mL, 4.0-4.9 um, 6x1 mL | 6x1mL | Spherotech |
CMPAK-5068-6K | Carboxyl Magnetic Blue Particle Array Kit, 6 peaks 107/mL 4.9-5.9 μm | 6x1mL | Spherotech |
CMX-300-10 | Carboxyl Magnetic Particles Crosslinked 1％ w/v 25-37 um 10 mL | 10mL | Spherotech |
VM-40-10 | Avidin Magnetic Particles, 1％w/v, 4.0-4.5µm, 10mL | 10mL | Spherotech |
CMX-40-10 | Carboxyl Magnetic Particles, Crosslinked, 2.5%w/v, 3.0-6.0µm, 10mL | 10mL | Spherotech |
ACURFP-38-5 | ACCUCOUNT ULTRARAINBOW FLUORESCENT PARTICLES | 5ml | Spherotech |
CP-15-10 | Carboxyl Polystyrene Particles, 5%w/v, 1.5-1.9um, 10mL | 10mL | Spherotech |
CP-20-10 | Carboxyl Polystyrene Particles, 5%w/v, 2.0-2.4um, 10mL | 10mL | Spherotech |
SVMS-30-10 | Streptavidin Magnetic Particles, Smooth, 1%w/v, 3.0-3.9µm, 10mL | 10mL | Spherotech |
SVP-100-4 | Streptavidin Polystyrene Particles, 0.5％ w/v, 10.0-14.0 um, 4 mL | 4mL | Spherotech |
CP-25-10 | Carboxyl Polystyrene Particles, 5%w/v, 2.5-2.9um, 10mL | 10mL | Spherotech |
SVMX-300-4 | Streptavidin Coated Magnetic Particles, Crosslinked 0.5％ w/v 25.0-37.0 µm | 4mL | Spherotech |
AM-05-10H | Amino Superparamagnetic Particles, High iron 1.0％w/v, 0.4-0.69um, 10mL | 10mL | Spherotech |
VP-10-10 | Avidin Polystyrene Particles, 5.0%w/v, 1.0-1.9µm, 10mL | 10mL | Spherotech |
VP-30-5 | Avidin Polystyrene Particles, 0.5%w/v, 3.0-3.9µm, 5mL | 5mL | Spherotech |
PGP-400-5 | Protein G Polystyrene Particles, 1％w/v, 38-52µm, 5mL | 5mL | Spherotech |
VP-60-5 | Avidin Polystyrene Particles, 0.5%w/v, 6.0-8.0µm, 5mL | 5mL | Spherotech |
